PROTEIN PRODUCTION, PURIFICATION, ANALYSIS
IBA offers a full complement of protein purification
services. IBA’s expertise allows us to independently
develop methods internally yet offer the flexability to
employ a procedure that is designed by you. Our goal is
to optimize your protein production through classic protein
purification methods or new and innovative strategies
for those challenging ligands. Protein purification is
a stand alone service or can be employed to complement
our assay method development, cell-based assay or antibody
production services. As a value-added service endotoxin
determination and removal as well as stable formulation
is available.
Purification Methods:
- Salt fractionation
- Column chromatography
-gel filtration
-ion exchange
-hydrophobicity
-size exclusion
- Affinity chromatography
-Ag or AB based
-protein A / G
-His-tag
- Differential centrifugation
- Preparative electrophoresis
- High-pressure Liquid Chromatography (HPLC)
- Reverse-phase Liquid Chromatography (RPLC)
Characterization Analysis:
- Denaturing electrophoresis (SDS-PAGE)
- Non-denaturing electrophoresis
- Western Blot
- ELISA
- Maldi-TOF
- N-terminal amino acid sequencing
- LC-MS/MS analysis of tryptic digests
- Carbohydrate Analysis (glycation)
Examples of Supported Sample Media
- Tissue culture supernatant
- Cell extract or homogenates
- Tissue extracts or homogenates
- Biological fluids (serum, plasma, urine, CSF, saliva,
BALF)
To contact an IBA representative call (203) 315-5972
or e-mail: info@iba-analytics.com
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