IBA offers a full complement of protein purification services. IBA’s expertise allows us to develop a method ourselves or to employ a procedure that is designed by you. Our goal is to optimize your protein production through classic protein purification methods or new and innovative strategies for those challenging ligands. Protein purification is a stand alone service or can be employed to complement our assay method development, cell-based assay or antibody production services. As a value-added service endotoxin determination and removal as well as stable formulation is available.

Purification Methods:

• Salt fractionation
• Column chromatography
  -gel filtration
  -ion exchange
  -hydrophobicity
  -size exclusion
• Affinity chromatography
  -Ag or AB based
  -protein A / G
  -His-tag
• Differential centrifugation
• Preparative electrophoresis
• High-pressure Liquid Chromatography (HPLC)
• Reverse-phase Liquid Chromatography (RPLC)

Characterization Analysis:

• Denaturing electrophoresis (SDS-PAGE)
• Non-denaturing electrophoresis
• Western Blot
• ELISA
• Maldi-TOF
• N-terminal amino acid sequencing
• LC-MS/MS analysis of tryptic digests
• Carbohydrate Analysis (glycation)

Examples of Supported Sample Media

• Tissue culture supernatant
• Cell extract or homogenates
• Tissue extracts or homogenates
• Biological fluids (serum, plasma, urine, CSF, saliva, BALF)

To contact an IBA representative call (203) 315-5972 or e-mail: info@iba-analytics.com